Crest Ultrasonic cleaning equipment is used to clean various types of contaminants such as oils, greases, particles, polishing compounds, protein, hemoglobin residues and many other contaminants from different components. In this study, Crest cleaning equipment were tested for its ability to kill bacterial endospores. So, this cleaner can be used where microbiological control is a major concern. This study was mainly conducted to check the effectiveness of various Ultrasonic and Megasonic frequencies on reduction of bacterial endospores such as Bacillus Atrophaeus and Geobacillus stearothermophilus. These bacterial endospores are used as a biological indicator to check the effectiveness of the sterilization process in medical, pharmaceutical, food and other industries.

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